swfchan: TRANSLATE20b.swf (info)

Text

Polyribosomes

Ribosome Cycle

A strand of mRNA is translated in the 5' to
3' direction from the start codon to the
stop codon.

Translation Overview

tRNA Charging

Initiation

Elongation

Termination

Small Ribosome Subunit
The small subunit of the ribosome
contains the decoding center in which
charged tRNAs are read the codon units of
the mRNA.

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tRNA
tRNA is the adaptor between a codon and
an amino acid. Each tRNA is charged with
a particular amino acid and each
recognizes a particular codon.

Large Ribosome Subunit
The large subunit of the ribosome contains
the peptidyl transferase center, which is
responsible for the formation of peptide
bonds.

IF3
Initiation Factor 3 (IF3) facilitates initiation
by binding to the small subunit and
preventing it from reattaching prematurely
to the large subunit.

IF2
Initiation Factor 2 (IF2) is a GTPase. It
interacts with IF1, fMettRNA and the
small ribosome subunit. IF2 facilitates
association of fMETtRNA and prevents
binding of other charged tRNAs to the
small subunit.

IF1
Initiation Factor 1 (IF1) facilitates initiation
by preventing tRNA from binding to the A
site of the small ribosome subunit.

EFTU
Elongation FactorTU (EFTu) is a GTPase
that is responsible for recruiting charged
aminoacyl tRNA to the A site of the
ribosome. EFTu can only bind to a
charged tRNA when it is associated with
GTP and releases the charged tRNA in
conjunction with the hydrolysis of GTP only
when there is a correct codon-anticodon
match.

EFG
Elongation factor G (EF-G) is a GTPase that
is responsible for translocating the
ribosome one codon unit along the mRNA
after each peptidyl transferase reaction.
GTP-bound EF-G binds to the A site of the
ribosome. Hydrolysis of GTP triggers the
movement of the ribosome relative to the
mRNA, thereby shifting the peptidyl tRNA
from the A site back to the P site.

Class II RF
The class II release factor causes the
class I RF to dissociate from the ribosome
after the polypeptide chain is released.

Class I RF
Class I Release Factors recognize stop
codons and trigger hydrolysis of the acyl
bondlinking the completed polypeptide
chain to the tRNA in the P site.

RRF
Ribosome Recycling Factor (RRF) mimics
tRNA by occupying the vacant A site after
polypeptide release. It helps release the P
and E site tRNAs by recruiting EFG to the
ribosome. Once the tRNAs are released it
dissociates from the ribosome.

Translation

mRNA

Start Codon

Stop Codon

Small Ribosomal Subunit

Charged tRNA

Translation begins with the binding of a free
small subunit and a charged tRNA to the
mRNA with the charged tRNA positioned over
the start codon.

Large Ribosomal Subunit

A large ribosomal subunit is recruited by the
mRNA/small subunit complex creating an
intact ribosome that is poised for translation.

Protein synthesis begins by the transfer of the
amino acid on the first charged tRNA to the
amino acid on the next incoming charged tRNA.

Protein synthesis continues with the
translocation of the ribosome to the next
codon on the mRNA and the transfer of the
growing polypeptide chain to the amino acid
on the next incoming charged tRNA.

As each codon goes by the newly synthesized
polypeptide grows.

Upon reaching a stop codon the peptide chain
is released and the subunits dissociate.

After dissociation, the small and large subunits
can re-associate at the start codon of the same
or a different mRNA and carry out another round
of protein synthesis. This cycle of association
and dissociation can be repeated multiple times
and is known as the ribosome cycle.

A ribosome is only capable of synthesizing a single
peptide strand at a time. A single mRNA can,
however, be translated simultaneously by a queue
of multiple ribosomes, each at a different stage of
completion of the growing polypeptide chain.

An mRNA that is being translated by multiple
ribosomes is known as a polysome.

Polyribosomes enable mulitiple polypeptides to be
generated from a single mRNA simultaneously.

Transfer to the tRNA

tRNA
tRNA is the adaptor between a codon
and an amino acid. Each tRNA is charged
with a particular amino acid and each
recognizes a particular codon.

Small Ribosomal Subunit
The small subunit of the ribosome
contains the decoding center in which
charged tRNAs are read the codon units of
the mRNA.

EFG
Primase is an RNA polymerase that
generates an oligoribonucleotide that is
extended by DNA polymerase III.

IF3
Blah, blah, blah....

EFTU
lhjasdfjaljksdhfb a;dflh amn a;lkj a
jkanzvnm v0w0u n lkjad

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Adenylylation

tRNA charging occurs in two steps,
adenylylation and transfer of the adenylylated
amino acid to tRNA. Adenylylation occurs when
an amino acid and ATP react. The amino acid is
adenylylated and pyrophosphate is released.

NH
2

Amino Acid

ATP

Adenylylated Amino Acid

Pyrophosphate

tRNA charging takes place when the
adenylylated amino acid reacts with tRNA,
which results in the release of AMP.

ACC

tRNA

AMP

High-energy Bond

CH
2

Both steps of tRNA charging are catalyzed by the
same enzyme (not depicted here), which is
called aminoacyl tRNA synthetase. Cells typically
have 20 synthetases, one for each amino acid.

Binding of mRNA and Initiator to the Small Subunit

Initiation Factors and the Initiation Complex

Creation of the First Peptide Bond

The ribosome is composed of large and
small subunits and has three binding sites
for tRNA called E, P, and A.

Small Subunit

Large Subunit

The A site binds charged tRNA.
The P site binds peptidyl tRNA.
The E site binds tRNA that had discharged the
growing polypeptide chain in the P site and is
about to be released from the ribosome.

In the initiation step of translation, mRNA binds
to the small subunit of the ribosome together
with a special charged tRNA called the initiator.
The initiator tRNA is charged with the unusual
amino acid N-formyl methionine (fMet).

UAC

fMet

UCCUC

AGGAG

AUG

Ribosome
Binding
Sequence

fMettRNA

CH
2

CH
3

The small subunit aligns itself on the mRNA
by base pairing between the ribosome
binding site in the message and a
complementary sequence near the 3' end of
the RNA component (called the 16S RNA) of
the small subunit.

Meanwhile, the charged initiator tRNA enters
what will become the P site of the ribosome
by base pairing between the start codon (AUG)
and the anticodon of the tRNA.

The preceding events are driven by three
initiation factors called IF1, IF2 and IF3.

Initiation factor 3 (IF3) attaches to the small
subunit. It binds in the area of the E site
preventing premature attachment of the
large subunit.

IF3

Initiation factor 1 (IF1) attaches to the small
subunit in the area of the A site blocking the
attachment of tRNA.

IF1

GTP

Initiation factor 2 is a GTPase. It interacts
with IF1, the small subunit and fMettRNA.

IF2

Once all the initiation factors are in place,
the small subunit can attach to the initiator
tRNA and mRNA.

fMet-tRNA

IF3 is released after the initiator and mRNA are
assembled on the small subunit, allowing the
large subunit to attach.

Binding of the large subunit leads to the
hydrolysis of GTP by IF2, reducing its affinity
for the ribosome and the initiator tRNA. This,
in turn, leads to the release of both IF2 and
IF1 from the ribosome.

GDP

The fully assembled ribosome with empty E
and A sites is known as the initiation
complex and is ready to accept a charged
tRNA into the A site and carry out the
formation of the first peptide bond.

NH
2

Aminoacyl-tRNA

An aminoacyl-tRNA with the correct anticodon
for the codon in the A site is recruited to the
A site by elongation factor EF-Tu (not shown
but introduced below).

Next, the ribosome catalyzes peptide bond
formation between the incoming amino acid
of the charged tRNA in the A site and the
fMet amino acid attached to the initiator
tRNA in the P site by bringing the 3’ ends of
the two charged tRNAs into close proximity.

The amino group of the A site aminoacyltRNA
attacks the carbonyl group of the fMettRNA,
creating the first peptide bond.

Elongation involves repeated cycles of the introduction
a charged tRNA into the A site, transfer of the growing
polypeptide chain in the P site to the amino acyl tRNA
in the A site, translocation of the ribosome along the
mRNA by one codon unit, and release of an uncharged
tRNA from the E site.

Introduction of Charged tRNA into the A site

Translocation

Peptidyl Transferase Reaction

Overview

As polypeptide elongation takes place,
aminoacyltRNAs are escorted to the A site
by elongation factor EFTu.

Peptidyl tRNA

AminoacyltRNA

Polypeptide Chain

EFTu

Factor Binding Center

EFTu is a GTPase and is only able to bind
to the aminoacyltRNA when it is associated
with GTP.

EFTuGTP

GTP-containing EF-Tu binds to aminoacyl-tRNA
and escorts it to the A site of the ribosome.
The GTPase is activated when EF-Tu contacts
the factor binding center and when a proper
codonanticodon match is made.

EFTu hydrolyzes its GTP and exits the ribosome
leaving the aminoacyltRNA in the A site.

In a cycle catalyzed by elongation factor EF-Ts
(not shown) , GDP is replaced by a fresh
molecule of GTP, re-generating GTP-containing
EF-Tu, which is poised to bind to a fresh
molecule of charged tRNA.

Peptide bond formation is catalyzed by the transfer
of the growing polypeptide chain in the P site to the
incoming aminoacyl tRNA in the A site. This is
called the peptidyl transferase reaction, and it is
catalyzed by an RNA enzyme (ribozyme) in the large
subunit of the ribosome (not shown).

As described above for the formation of the first
peptide bond, the peptidyl transferase reaction
takes place by attack of the amino group of the
aminoacyl tRNA in the A site on the carbonyl
group of the peptidyl tRNA in the P site.

Peptidyl-tRNA

In the process of bond formation, the growing
polypeptide chain is transferred from the
peptidyl-tRNA to the aminoacyltRNA.

Translocation of the ribosome along the
mRNA is catalyzed by elongation factor EF-G,
which, like EF-Tu, is a GTPase.

EFG

GTP hydrolysis by GTP-containing EF-G causes the
ribosome to advance one codon unit along the
mRNA, thereby shifting the peptidyl tRNA from the
previous peptidyl transferase reaction back to the
P site and shifting the deacylated tRNA in the P site
to the E site, where it exits the ribosome.

After translocation, EF-G-GDP dissociates
from the ribosome and its cargo of GDP is
replaced by a fresh molecule of GTP.

Cycles of peptide bond formation and translocation
continue until a stop codon is reached.

Esite tRNA

Ribosome Dissociation

Polypeptide Release

The stop codon is recognized by release
factors (RFs), which cause the release of the
now complete polypeptide chain from the
peptidyl-tRNA in the P site.

Class I RF

A class I release factor recognizes the stop
codon with a peptide anticodon at one end of the
molecule (highlighted in blue). A domain at the
other end of the release factor (green) triggers
the hydrolysis of the acyl bond to the tRNA.

Next, a class II release factor triggers the
release of the class I release factor from the
ribosome in a process involving the exchange
of GTP for GDP on the factor and its
subsequent hydrolysis.

Class II RF

A final series of events involving a ribosome
recycling factor (RRF), EF-G and IF-3 causes
the discharge of the mRNA and deacylated
tRNAs from the ribosome and the dissociation
of the ribosome into its subunits, with IF-3
bound to the small subunit.

Deacylated tRNA

RRF

EFGGTP

The IF3-bound small subunit is now poised
to initiaite a new cycle of protein synthesis.

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Symbol 127 Graphic	Used by:Timeline
Symbol 128 Text	Uses:69	Used by:Timeline
Symbol 129 Text	Uses:69	Used by:Timeline
Symbol 130 Text	Uses:69	Used by:Timeline
Symbol 131 Text	Uses:69	Used by:Timeline
Symbol 132 Text	Uses:69	Used by:Timeline
Symbol 133 Graphic	Used by:Timeline
Symbol 134 Text	Uses:125	Used by:Timeline
Symbol 135 Text	Uses:125	Used by:Timeline
Symbol 136 Graphic	Used by:Timeline
Symbol 137 Text	Uses:69	Used by:Timeline
Symbol 138 Text	Uses:69	Used by:Timeline
Symbol 139 Text	Uses:15	Used by:Timeline
Symbol 140 Graphic	Used by:Timeline
Symbol 141 Graphic	Used by:Timeline
Symbol 142 Graphic	Used by:Timeline
Symbol 143 Graphic	Used by:Timeline
Symbol 144 Graphic	Used by:Timeline
Symbol 145 Graphic	Used by:Timeline
Symbol 146 Graphic	Used by:Timeline
Symbol 147 Text	Uses:69	Used by:Timeline
Symbol 148 Text	Uses:15	Used by:Timeline
Symbol 149 Graphic	Used by:Timeline
Symbol 150 Graphic	Used by:Timeline
Symbol 151 Text	Uses:15	Used by:Timeline
Symbol 152 Text	Uses:15	Used by:Timeline
Symbol 153 Graphic	Used by:Timeline
Symbol 154 Graphic	Used by:Timeline
Symbol 155 Graphic	Used by:Timeline
Symbol 156 Graphic	Used by:Timeline
Symbol 157 Text	Uses:15	Used by:Timeline
Symbol 158 Text	Uses:15	Used by:Timeline
Symbol 159 Text	Uses:15	Used by:Timeline
Symbol 160 Graphic	Used by:Timeline
Symbol 161 Graphic	Used by:Timeline
Symbol 162 Graphic	Used by:Timeline
Symbol 163 Text	Uses:15	Used by:Timeline
Symbol 164 Graphic	Used by:Timeline
Symbol 165 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 166 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 167 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 168 Graphic	Used by:Timeline
Symbol 169 Button	Uses:63 64 65	Used by:Timeline
Symbol 170 Text	Uses:15	Used by:Timeline
Symbol 171 Graphic	Used by:Timeline
Symbol 172 ShapeTweening	Used by:Timeline
Symbol 173 ShapeTweening	Used by:Timeline
Symbol 174 ShapeTweening	Used by:Timeline
Symbol 175 ShapeTweening	Used by:Timeline
Symbol 176 ShapeTweening	Used by:Timeline
Symbol 177 ShapeTweening	Used by:Timeline
Symbol 178 ShapeTweening	Used by:Timeline
Symbol 179 ShapeTweening	Used by:Timeline
Symbol 180 Graphic	Used by:Timeline
Symbol 181 ShapeTweening	Used by:Timeline
Symbol 182 ShapeTweening	Used by:Timeline
Symbol 183 ShapeTweening	Used by:Timeline
Symbol 184 ShapeTweening	Used by:Timeline
Symbol 185 ShapeTweening	Used by:Timeline
Symbol 186 ShapeTweening	Used by:Timeline
Symbol 187 ShapeTweening	Used by:Timeline
Symbol 188 ShapeTweening	Used by:Timeline
Symbol 189 ShapeTweening	Used by:Timeline
Symbol 190 ShapeTweening	Used by:Timeline
Symbol 191 ShapeTweening	Used by:Timeline
Symbol 192 Text	Uses:15	Used by:Timeline
Symbol 193 ShapeTweening	Used by:Timeline
Symbol 194 ShapeTweening	Used by:Timeline
Symbol 195 ShapeTweening	Used by:Timeline
Symbol 196 ShapeTweening	Used by:Timeline
Symbol 197 ShapeTweening	Used by:Timeline
Symbol 198 ShapeTweening	Used by:Timeline
Symbol 199 ShapeTweening	Used by:Timeline
Symbol 200 ShapeTweening	Used by:Timeline
Symbol 201 ShapeTweening	Used by:Timeline
Symbol 202 ShapeTweening	Used by:Timeline
Symbol 203 ShapeTweening	Used by:Timeline
Symbol 204 ShapeTweening	Used by:Timeline
Symbol 205 ShapeTweening	Used by:Timeline
Symbol 206 ShapeTweening	Used by:Timeline
Symbol 207 ShapeTweening	Used by:Timeline
Symbol 208 ShapeTweening	Used by:Timeline
Symbol 209 Graphic	Used by:Timeline
Symbol 210 EditableText	Uses:2	Used by:213 245 288
Symbol 211 EditableText	Uses:2	Used by:213 245 288
Symbol 212 EditableText	Uses:2	Used by:213 245 288
Symbol 213 Button	Uses:210 211 212 6	Used by:Timeline
Symbol 214 Button	Uses:3 4 5 6	Used by:Timeline
Symbol 215 Graphic	Used by:Timeline
Symbol 216 Button	Used by:Timeline
Symbol 217 Graphic	Used by:220
Symbol 218 Text	Uses:69 15	Used by:220
Symbol 219 Graphic	Used by:220 228
Symbol 220 Button	Uses:217 218 219	Used by:Timeline
Symbol 221 Graphic	Used by:225
Symbol 222 Text	Uses:69 15	Used by:225
Symbol 223 Graphic	Used by:225
Symbol 224 Graphic	Used by:225
Symbol 225 Button	Uses:67 221 222 223 224	Used by:Timeline
Symbol 226 Graphic	Used by:228
Symbol 227 Text	Uses:69 15	Used by:228
Symbol 228 Button	Uses:106 226 227 219	Used by:Timeline
Symbol 229 Graphic	Used by:232
Symbol 230 Text	Uses:69 15	Used by:232
Symbol 231 Graphic	Used by:232 235
Symbol 232 Button	Uses:86 229 230 231	Used by:Timeline
Symbol 233 Graphic	Used by:235
Symbol 234 Text	Uses:69 15	Used by:235
Symbol 235 Button	Uses:101 233 234 231	Used by:Timeline
Symbol 236 Button	Uses:46 47 48 49	Used by:Timeline
Symbol 237 Button	Uses:51 52 53	Used by:Timeline
Symbol 238 Button	Uses:55 56 57	Used by:Timeline
Symbol 239 Text	Uses:2 73 74	Used by:Timeline
Symbol 240 Graphic	Used by:Timeline
Symbol 241 EditableText	Uses:2	Used by:244
Symbol 242 EditableText	Uses:2	Used by:244
Symbol 243 EditableText	Uses:2	Used by:244
Symbol 244 Button	Uses:241 242 243 6	Used by:Timeline
Symbol 245 Button	Uses:210 211 212 6	Used by:Timeline
Symbol 246 Graphic	Used by:Timeline
Symbol 247 Text	Uses:15	Used by:Timeline
Symbol 248 Graphic	Used by:Timeline
Symbol 249 Text	Uses:69	Used by:Timeline
Symbol 250 Text	Uses:15	Used by:Timeline
Symbol 251 Text	Uses:15	Used by:Timeline
Symbol 252 Text	Uses:15	Used by:Timeline
Symbol 253 Text	Uses:15	Used by:Timeline
Symbol 254 Text	Uses:15	Used by:Timeline
Symbol 255 Text	Uses:69	Used by:Timeline
Symbol 256 Graphic	Used by:Timeline
Symbol 257 Text	Uses:69	Used by:Timeline
Symbol 258 Text	Uses:15	Used by:Timeline
Symbol 259 Text	Uses:15	Used by:Timeline
Symbol 260 Text	Uses:15	Used by:Timeline
Symbol 261 Text	Uses:69	Used by:Timeline
Symbol 262 Text	Uses:69	Used by:Timeline
Symbol 263 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 264 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 265 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 266 Graphic	Used by:Timeline
Symbol 267 Graphic	Used by:Timeline
Symbol 268 ShapeTweening	Used by:Timeline
Symbol 269 ShapeTweening	Used by:Timeline
Symbol 270 ShapeTweening	Used by:Timeline
Symbol 271 ShapeTweening	Used by:Timeline
Symbol 272 Graphic	Used by:Timeline
Symbol 273 Graphic	Used by:Timeline
Symbol 274 Graphic	Used by:Timeline
Symbol 275 Graphic	Used by:Timeline
Symbol 276 Graphic	Used by:Timeline
Symbol 277 Text	Uses:69	Used by:Timeline
Symbol 278 Text	Uses:69	Used by:Timeline
Symbol 279 Graphic	Used by:Timeline
Symbol 280 Graphic	Used by:Timeline
Symbol 281 Text	Uses:69	Used by:Timeline
Symbol 282 Text	Uses:15	Used by:Timeline
Symbol 283 Graphic	Used by:Timeline
Symbol 284 Text	Uses:69	Used by:Timeline
Symbol 285 Text	Uses:69	Used by:Timeline
Symbol 286 Text	Uses:15	Used by:Timeline
Symbol 287 Graphic	Used by:Timeline
Symbol 288 Button	Uses:210 211 212 6	Used by:Timeline
Symbol 289 Text	Uses:69	Used by:Timeline
Symbol 290 Graphic	Used by:Timeline
Symbol 291 ShapeTweening	Used by:Timeline
Symbol 292 ShapeTweening	Used by:Timeline
Symbol 293 ShapeTweening	Used by:Timeline
Symbol 294 Graphic	Used by:Timeline
Symbol 295 Graphic	Used by:Timeline
Symbol 296 Graphic	Used by:Timeline
Symbol 297 Text	Uses:15	Used by:Timeline
Symbol 298 Graphic	Used by:Timeline
Symbol 299 Text	Uses:69 15	Used by:Timeline
Symbol 300 Graphic	Used by:Timeline
Symbol 301 Graphic	Used by:Timeline
Symbol 302 Text	Uses:69	Used by:Timeline
Symbol 303 Text	Uses:15	Used by:Timeline
Symbol 304 Graphic	Used by:Timeline
Symbol 305 Graphic	Used by:Timeline
Symbol 306 Graphic	Used by:Timeline
Symbol 307 Graphic	Used by:Timeline
Symbol 308 Text	Uses:15	Used by:Timeline
Symbol 309 Text	Uses:15	Used by:Timeline
Symbol 310 Text	Uses:15	Used by:Timeline
Symbol 311 Graphic	Used by:Timeline
Symbol 312 Graphic	Used by:Timeline
Symbol 313 Graphic	Used by:Timeline
Symbol 314 Text	Uses:15	Used by:Timeline
Symbol 315 Graphic	Used by:Timeline
Symbol 316 EditableText	Uses:2	Used by:319
Symbol 317 EditableText	Uses:2	Used by:319
Symbol 318 EditableText	Uses:2	Used by:319
Symbol 319 Button	Uses:316 317 318 6	Used by:Timeline
Symbol 320 EditableText	Uses:2	Used by:323
Symbol 321 EditableText	Uses:2	Used by:323
Symbol 322 EditableText	Uses:2	Used by:323
Symbol 323 Button	Uses:320 6 321 322	Used by:Timeline
Symbol 324 EditableText	Uses:2	Used by:327
Symbol 325 EditableText	Uses:2	Used by:327
Symbol 326 EditableText	Uses:2	Used by:327
Symbol 327 Button	Uses:324 6 325 326	Used by:Timeline
Symbol 328 Text	Uses:15 69	Used by:Timeline
Symbol 329 Graphic	Used by:Timeline
Symbol 330 Graphic	Used by:Timeline
Symbol 331 Graphic	Used by:Timeline
Symbol 332 Text	Uses:69	Used by:Timeline
Symbol 333 Text	Uses:69	Used by:Timeline
Symbol 334 Text	Uses:69	Used by:Timeline
Symbol 335 Text	Uses:69	Used by:Timeline
Symbol 336 Text	Uses:69	Used by:Timeline
Symbol 337 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 338 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 339 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 340 Graphic	Used by:Timeline
Symbol 341 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 342 Text	Uses:15 69	Used by:Timeline
Symbol 343 Text	Uses:15	Used by:Timeline
Symbol 344 Graphic	Used by:Timeline
Symbol 345 Text	Uses:69	Used by:Timeline
Symbol 346 Text	Uses:69	Used by:Timeline
Symbol 347 Text	Uses:69	Used by:Timeline
Symbol 348 Graphic	Used by:Timeline
Symbol 349 Text	Uses:69	Used by:Timeline
Symbol 350 Text	Uses:69	Used by:Timeline
Symbol 351 Text	Uses:69	Used by:Timeline
Symbol 352 Graphic	Used by:Timeline
Symbol 353 Text	Uses:69	Used by:Timeline
Symbol 354 Text	Uses:69	Used by:Timeline
Symbol 355 Graphic	Used by:Timeline
Symbol 356 Text	Uses:69	Used by:Timeline
Symbol 357 Text	Uses:69	Used by:Timeline
Symbol 358 ShapeTweening	Used by:Timeline
Symbol 359 Graphic	Used by:Timeline
Symbol 360 Graphic	Used by:Timeline
Symbol 361 Graphic	Used by:Timeline
Symbol 362 Graphic	Used by:Timeline
Symbol 363 Graphic	Used by:Timeline
Symbol 364 Text	Uses:15	Used by:Timeline
Symbol 365 Text	Uses:15	Used by:Timeline
Symbol 366 Text	Uses:15	Used by:Timeline
Symbol 367 Text	Uses:15	Used by:Timeline
Symbol 368 Graphic	Used by:Timeline
Symbol 369 Graphic	Used by:Timeline
Symbol 370 Text	Uses:15	Used by:Timeline
Symbol 371 Text	Uses:15	Used by:Timeline
Symbol 372 Text	Uses:15	Used by:Timeline
Symbol 373 Text	Uses:69	Used by:Timeline
Symbol 374 Text	Uses:69	Used by:Timeline
Symbol 375 Text	Uses:69	Used by:Timeline
Symbol 376 Text	Uses:69	Used by:Timeline
Symbol 377 Text	Uses:69	Used by:Timeline
Symbol 378 Text	Uses:69	Used by:Timeline
Symbol 379 Graphic	Used by:Timeline
Symbol 380 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 381 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 382 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 383 Graphic	Used by:Timeline
Symbol 384 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 385 Text	Uses:15	Used by:Timeline
Symbol 386 Graphic	Used by:Timeline
Symbol 387 Text	Uses:69	Used by:Timeline
Symbol 388 Graphic	Used by:Timeline
Symbol 389 Text	Uses:15	Used by:Timeline
Symbol 390 Graphic	Used by:Timeline
Symbol 391 Text	Uses:69	Used by:Timeline
Symbol 392 Graphic	Used by:Timeline
Symbol 393 Text	Uses:125	Used by:Timeline
Symbol 394 Graphic	Used by:Timeline
Symbol 395 Text	Uses:69	Used by:Timeline
Symbol 396 Text	Uses:69	Used by:Timeline
Symbol 397 Text	Uses:15	Used by:Timeline
Symbol 398 Graphic	Used by:Timeline
Symbol 399 Text	Uses:69	Used by:Timeline
Symbol 400 Text	Uses:15	Used by:Timeline
Symbol 401 Graphic	Used by:Timeline
Symbol 402 Text	Uses:69	Used by:Timeline
Symbol 403 ShapeTweening	Used by:Timeline
Symbol 404 ShapeTweening	Used by:Timeline
Symbol 405 Graphic	Used by:Timeline
Symbol 406 Graphic	Used by:Timeline
Symbol 407 ShapeTweening	Used by:Timeline
Symbol 408 ShapeTweening	Used by:Timeline
Symbol 409 Graphic	Used by:Timeline
Symbol 410 Text	Uses:15	Used by:Timeline
Symbol 411 Graphic	Used by:Timeline
Symbol 412 Text	Uses:15	Used by:Timeline
Symbol 413 Graphic	Used by:Timeline
Symbol 414 Text	Uses:69	Used by:Timeline
Symbol 415 Text	Uses:69	Used by:Timeline
Symbol 416 Text	Uses:15	Used by:Timeline
Symbol 417 Graphic	Used by:Timeline
Symbol 418 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 419 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 420 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 421 Graphic	Used by:Timeline
Symbol 422 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 423 ShapeTweening	Used by:Timeline
Symbol 424 ShapeTweening	Used by:Timeline
Symbol 425 Graphic	Used by:Timeline
Symbol 426 Text	Used by:Timeline
Symbol 427 Graphic	Used by:Timeline
Symbol 428 Graphic	Used by:Timeline
Symbol 429 Graphic	Used by:Timeline
Symbol 430 Graphic	Used by:Timeline
Symbol 431 Graphic	Used by:Timeline
Symbol 432 Text	Uses:15	Used by:Timeline
Symbol 433 Graphic	Used by:Timeline
Symbol 434 Text	Uses:15	Used by:Timeline
Symbol 435 Text	Uses:15	Used by:Timeline
Symbol 436 Text	Uses:15	Used by:Timeline
Symbol 437 Text	Uses:15	Used by:Timeline
Symbol 438 Text	Uses:69	Used by:Timeline
Symbol 439 Text	Uses:15	Used by:Timeline
Symbol 440 Text	Uses:15	Used by:Timeline
Symbol 441 Graphic	Used by:Timeline
Symbol 442 Text	Uses:15	Used by:Timeline
Symbol 443 Graphic	Used by:Timeline
Symbol 444 Graphic	Used by:Timeline
Symbol 445 Text	Uses:15	Used by:Timeline
Symbol 446 Graphic	Used by:Timeline
Symbol 447 Graphic	Used by:Timeline
Symbol 448 Graphic	Used by:Timeline
Symbol 449 Text	Uses:15	Used by:Timeline
Symbol 450 Graphic	Used by:Timeline
Symbol 451 Graphic	Used by:Timeline
Symbol 452 Graphic	Used by:Timeline
Symbol 453 Text	Uses:15	Used by:Timeline
Symbol 454 Graphic	Used by:Timeline
Symbol 455 EditableText	Uses:2	Used by:458
Symbol 456 EditableText	Uses:2	Used by:458
Symbol 457 EditableText	Uses:2	Used by:458
Symbol 458 Button	Uses:455 456 457 6	Used by:Timeline
Symbol 459 EditableText	Uses:2	Used by:462
Symbol 460 EditableText	Uses:2	Used by:462
Symbol 461 EditableText	Uses:2	Used by:462
Symbol 462 Button	Uses:459 460 461 6	Used by:Timeline
Symbol 463 EditableText	Uses:2	Used by:466
Symbol 464 EditableText	Uses:2	Used by:466
Symbol 465 EditableText	Uses:2	Used by:466
Symbol 466 Button	Uses:463 6 464 465	Used by:Timeline
Symbol 467 EditableText	Uses:2	Used by:470
Symbol 468 EditableText	Uses:2	Used by:470
Symbol 469 EditableText	Uses:2	Used by:470
Symbol 470 Button	Uses:467 468 469 6	Used by:Timeline
Symbol 471 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 472 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 473 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 474 Graphic	Used by:Timeline
Symbol 475 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 476 ShapeTweening	Used by:Timeline
Symbol 477 Graphic	Used by:Timeline
Symbol 478 Graphic	Used by:Timeline
Symbol 479 Graphic	Used by:Timeline
Symbol 480 Graphic	Used by:Timeline
Symbol 481 Text	Uses:15	Used by:Timeline
Symbol 482 Graphic	Used by:Timeline
Symbol 483 Graphic	Used by:Timeline
Symbol 484 Graphic	Used by:Timeline
Symbol 485 Text	Uses:69	Used by:Timeline
Symbol 486 Text	Uses:69	Used by:Timeline
Symbol 487 Text	Uses:69	Used by:Timeline
Symbol 488 Graphic	Used by:Timeline
Symbol 489 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 490 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 491 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 492 Graphic	Used by:Timeline
Symbol 493 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 494 Graphic	Used by:Timeline
Symbol 495 Text	Uses:69	Used by:Timeline
Symbol 496 Text	Uses:69	Used by:Timeline
Symbol 497 Text	Uses:15	Used by:Timeline
Symbol 498 Graphic	Used by:Timeline
Symbol 499 Text	Uses:69	Used by:Timeline
Symbol 500 Text	Uses:15	Used by:Timeline
Symbol 501 Text	Uses:15	Used by:Timeline
Symbol 502 Text	Uses:15	Used by:Timeline
Symbol 503 Graphic	Used by:Timeline
Symbol 504 Graphic	Used by:Timeline
Symbol 505 Text	Uses:15	Used by:Timeline
Symbol 506 Graphic	Used by:Timeline
Symbol 507 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 508 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 509 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 510 Graphic	Used by:Timeline
Symbol 511 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 512 Graphic	Used by:Timeline
Symbol 513 ShapeTweening	Used by:Timeline
Symbol 514 ShapeTweening	Used by:Timeline
Symbol 515 Graphic	Used by:Timeline
Symbol 516 Graphic	Used by:Timeline
Symbol 517 Text	Uses:15	Used by:Timeline
Symbol 518 Graphic	Used by:Timeline
Symbol 519 Graphic	Used by:Timeline
Symbol 520 Graphic	Used by:Timeline
Symbol 521 Text	Uses:15	Used by:Timeline
Symbol 522 Text	Uses:69	Used by:Timeline
Symbol 523 Graphic	Used by:Timeline
Symbol 524 Graphic	Used by:Timeline
Symbol 525 Graphic	Used by:Timeline
Symbol 526 Graphic	Used by:Timeline
Symbol 527 Graphic	Used by:Timeline
Symbol 528 Graphic	Used by:Timeline
Symbol 529 Text	Uses:15	Used by:Timeline
Symbol 530 Graphic	Used by:Timeline
Symbol 531 Text	Uses:15	Used by:Timeline
Symbol 532 Graphic	Used by:Timeline
Symbol 533 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 534 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 535 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 536 Graphic	Used by:Timeline
Symbol 537 Graphic	Used by:Timeline
Symbol 538 Text	Uses:69	Used by:Timeline
Symbol 539 Text	Uses:15	Used by:Timeline
Symbol 540 Text	Uses:15	Used by:Timeline
Symbol 541 Text	Uses:15	Used by:Timeline
Symbol 542 Graphic	Used by:Timeline
Symbol 543 Text	Uses:69	Used by:Timeline
Symbol 544 Graphic	Used by:Timeline
Symbol 545 EditableText	Uses:2	Used by:548
Symbol 546 EditableText	Uses:2	Used by:548
Symbol 547 EditableText	Uses:2	Used by:548
Symbol 548 Button	Uses:545 546 547 6	Used by:Timeline
Symbol 549 EditableText	Uses:2	Used by:552
Symbol 550 EditableText	Uses:2	Used by:552
Symbol 551 EditableText	Uses:2	Used by:552
Symbol 552 Button	Uses:549 550 551 6	Used by:Timeline
Symbol 553 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 554 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 555 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 556 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 557 Text	Uses:15	Used by:Timeline
Symbol 558 Graphic	Used by:Timeline
Symbol 559 Text	Uses:69	Used by:Timeline
Symbol 560 Text	Uses:15	Used by:Timeline
Symbol 561 Graphic	Used by:Timeline
Symbol 562 Graphic	Used by:Timeline
Symbol 563 Text	Uses:15	Used by:Timeline
Symbol 564 Graphic	Used by:Timeline
Symbol 565 Text	Uses:69	Used by:Timeline
Symbol 566 Text	Uses:15	Used by:Timeline
Symbol 567 Graphic	Used by:Timeline
Symbol 568 Text	Uses:69	Used by:Timeline
Symbol 569 Graphic	Used by:Timeline
Symbol 570 Button	Uses:18 19 20 21	Used by:Timeline
Symbol 571 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 572 Button	Uses:38 39 40 41	Used by:Timeline
Symbol 573 Graphic	Used by:Timeline
Symbol 574 Button	Uses:33 34 35 36	Used by:Timeline
Symbol 575 Graphic	Used by:Timeline
Symbol 576 Text	Uses:69	Used by:Timeline
Symbol 577 Graphic	Used by:Timeline
Symbol 578 Graphic	Used by:Timeline
Symbol 579 Text	Uses:69	Used by:Timeline
Symbol 580 Graphic	Used by:Timeline
Symbol 581 Graphic	Used by:Timeline
Symbol 582 Graphic	Used by:Timeline
Symbol 583 Graphic	Used by:Timeline
Symbol 584 Graphic	Used by:Timeline
Symbol 585 Text	Uses:15	Used by:Timeline

Special Tags

Protect (24)

Timeline Frame 1

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Labels

"ribosome Cycle"	Frame 1
"polyribosomes"	Frame 2044
"adenylylation"	Frame 2828
"tRNAcharging"	Frame 3120
"Ribo binding site"	Frame 3845
"assembly start"	Frame 4967
"end assembly"	Frame 6575
"first bond start"	Frame 6576
"elongation start"	Frame 7344
"elongation2 start"	Frame 8620
"PTR start"	Frame 9524
"elongation3 start"	Frame 10278
"elongation4 start"	Frame 10857
"termination start"	Frame 12025